Dr. Nazish Annum
Wheat Biotechnology Lab
Contact Information: 0092-323-6814398 annum21naz@gmail.com
For Detail CV click here: 
I possess profound expertise in genetic engineering, genome editing, bioinformatics, plant breeding, and various In vitro and In vivo techniques, including radioactive lipid profiling. My professional aspirations are to integrate these fields to develop crop varieties that are resilient to environmental changes. In my prior research at NIBGE and SILS, University of Amsterdam (UvA), The Netherlands, I investigated the role of the AtPLC5 gene in thermo-tolerance in Arabidopsis thaliana. I successfully cloned and overexpressed the AtPLC5 gene in a local wheat cultivar to enhance heat tolerance. Additionally, I used 32Pi radioactive isotope labeling to study the responses of Phosphatidylinositol 4,5-bisphosphate (PIP2) and Phosphatidic Acid (PA) under heat and osmotic stress in transgenic wheat. I also worked as a Research Associate on the PSF/NSLP Project # 960 entitled “CRISPR-Cas9 based precise excision of TaHyPRP1 domain(s) to generate salt stress tolerance in wheat.” Currently, at the Center for Advanced Studies in Agriculture and Food Sciences (CAS-AFS), I focus on the genome editing of cereals, particularly wheat, to improve resistant starch, grain weight, and iron and zinc contents. In oilseed crops, I work on brassica to enhance the oil profile by reducing erucic acid content, addressing the challenges posed by climate change.
Publications
(1). • Annum N, Ahmed M, Tester M, Mukhtar Z and Saeed NA (2023). Physiological responses induced by phospholipase C isoform 5 upon heat stress in Arabidopsis thaliana. Front. Plant Sci. 14:1076331. http://doi:10.3389/fpls.2023.1076331. http://doi:10.3389/fpls.2023.1076331
(2). • Imtiaz K, Ahmed M, Annum N, Tester M and Saeed NA (2023). AtCIPK16, a CBL-Interacting Protein Kinase Gene confers salinity Tolerance in Transgenic Wheat. Front. Plant Sci. 14:1127311. https://doi.org/10.3389/fpls.2023.1127311 https://doi.org/10.3389/fpls.2023.1127311
(3). • Annum N, Ahmed M, Imtiaz K, Mansoor S, Tester M and Saeed NA (2022). 32Pi Labeled Transgenic Wheat Shows the Accumulation of Phosphatidylinositol 4,5-bisphosphate and Phosphatidic Acid Under Heat and Osmotic Stress. Front. Plant Sci. 13:881188. https://doi.org/10.3389/fpls.2022.881188 https://doi.org/10.3389/fpls.2022.881188
I possess profound expertise in genetic engineering, genome editing, bioinformatics, plant breeding, and various In vitro and In vivo techniques, including radioactive lipid profiling. My professional aspirations are to integrate these fields to develop crop varieties that are resilient to environmental changes. In my prior research at NIBGE and SILS, University of Amsterdam (UvA), The Netherlands, I investigated the role of the AtPLC5 gene in thermo-tolerance in Arabidopsis thaliana. I successfully cloned and overexpressed the AtPLC5 gene in a local wheat cultivar to enhance heat tolerance. Additionally, I used 32Pi radioactive isotope labeling to study the responses of Phosphatidylinositol 4,5-bisphosphate (PIP2) and Phosphatidic Acid (PA) under heat and osmotic stress in transgenic wheat. I also worked as a Research Associate on the PSF/NSLP Project # 960 entitled “CRISPR-Cas9 based precise excision of TaHyPRP1 domain(s) to generate salt stress tolerance in wheat.” Currently, at the Center for Advanced Studies in Agriculture and Food Sciences (CAS-AFS), I focus on the genome editing of cereals, particularly wheat, to improve resistant starch, grain weight, and iron and zinc contents. In oilseed crops, I work on brassica to enhance the oil profile by reducing erucic acid content, addressing the challenges posed by climate change.
Publications
(1). • Annum N, Ahmed M, Tester M, Mukhtar Z and Saeed NA (2023). Physiological responses induced by phospholipase C isoform 5 upon heat stress in Arabidopsis thaliana. Front. Plant Sci. 14:1076331. http://doi:10.3389/fpls.2023.1076331. http://doi:10.3389/fpls.2023.1076331
(2). • Imtiaz K, Ahmed M, Annum N, Tester M and Saeed NA (2023). AtCIPK16, a CBL-Interacting Protein Kinase Gene confers salinity Tolerance in Transgenic Wheat. Front. Plant Sci. 14:1127311. https://doi.org/10.3389/fpls.2023.1127311 https://doi.org/10.3389/fpls.2023.1127311
(3). • Annum N, Ahmed M, Imtiaz K, Mansoor S, Tester M and Saeed NA (2022). 32Pi Labeled Transgenic Wheat Shows the Accumulation of Phosphatidylinositol 4,5-bisphosphate and Phosphatidic Acid Under Heat and Osmotic Stress. Front. Plant Sci. 13:881188. https://doi.org/10.3389/fpls.2022.881188 https://doi.org/10.3389/fpls.2022.881188